- 纯化技术 -
The booming field of biologics places ever greater demands on protein production. Whether for research or to create biotherapeutics, scientists must purify protein following expression and scale-up.  To streamline processes, researchers need to integrate upstream and downstream phases, and reduce the number of steps required to achieve pure, properly-folded protein. A plethora of technologies and tools are available to support purification, but which are the best? As protein-based molecules become more complicated, they pose greater challenges for purification. Bispecifics, ADCs, fusion and membrane proteins present purification obstacles that may not have been tackled before. And aggregation continues to be a nagging issue waiting to trip up an earnest researcher. This conference presents the tricks and technologies used by purification leaders who have refined techniques that remove headaches from protein processing. Experts will address ‘traditional’ technologies as well as new approaches, and will touch on issues of scale, as well as purifying protein in various expression systems; all with an eye to streamline purification while ensuring quality.


Preliminary Agenda

IMPROVING PURIFICATION PROCESSES

KEYNOTE PRESENTATION: Streamlining the Production of Recombinant Proteins for Structural Proteomics: One Size Fits All?
Ray Owens, PhD, Professor, Molecular Biology and Head, Oxford Protein Production Facility, University of Oxford

Simplification of Purification Strategies for Mammalian Proteins from Single Targets to High-Throughput Projects Using an Enhanced Biochemical Code
David O'Connell, PhD, Lecturer, Biotherapeutics, Biomolecular & Biomedical Science, University College Dublin

HARNESSING THE POWER OF SMALL

Purification of Fabs from E. coli Cell Lysates – A Tricky Endeavour
Oliver Spadiut, PhD, Assistant Professor, Chemical, Environmental and Biological Engineering, Integrated Bioprocess Development, Technische Universität Wien (TU Wien)

Protein Purification and Detection with Nanobodies
Ulrich Rothbauer, PhD, Professor, Pharmaceutical Biotechnology, Natural and Medical Sciences Institute, University of Tübingen

NEXT-GEN CHROMATOGRAPHY

Potential of Centrifugal Partition Chromatography for the Separation of Proteins
Mirjana Minceva, PhD, Assistant Professor, Biothermodynamics, Life and Food Sciences Weihenstephan, Technische Universität München

Mechanical Characterisation of Agarose-Based Chromatography Resins
Graham McCartney, PhD, Platform Lead, Downstream Processing, Eli Lilly and Company

Nanofibre Based Protein a Chromatography for Truly Disposable Chromatography
Daniel Bracewell, PhD, Professor, Bioprocess Analysis, Biochemical Engineering, University College London

PURIFYING MEMBRANE PROTEINS

Structural Biology of Membrane Proteins: Expression Tricks, and to Solubilize or Not to Solubilize
Dirk Linke, PhD, Professor, Genetics and Evolutionary Biology, Biosciences, University of Oslo

Adaptive Automated Membrane Protein Purification using AkTA Avant
Jonas Lee, PhD, Scientist, Protein Technologies, Amgen, Inc.

Nanoparticles to Stabilize Membrane Proteins in a Lipid Environment
Jens Frauenfeld, PhD, CEO, Salipro Biotech AB

SCALING UP PURIFICATION PROCESSES AND MANUFACTURING

Pitfalls in Design and Scaling Up Protein Chromatography
Dorota Antos, PhD, Professor, Chemical and Process Engineering, Rzeszow University of Technology

Monoclonal Antibody Reduction and Re-Oxidation by Copper Sulfate During Manufacturing and Impact on Product Quality
Green Guihang Zhang, PhD, Associate Director, Large Molecule Purification Development, Global Product and Process Development, Incyte Corporation


* 活动内容有可能不事先告知作更动及调整。

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