Cambridge Healthtech Institute’s 5th Annual

Protein Expression System Engineering
( 蛋白质比现系统工程 )

Gene to Cell Line

2019年4月11~12日

 

CHI’s 5th annual Protein Expression System Engineering conference examines the functioning of the cellular machinery harnessed during protein biosynthesis and how to engineer hosts to efficiently express a protein of interest. The intricate steps required to achieve properly folded protein will be discussed, including verification and sequence analysis of the gene, codon optimization, vector construction, selecting and optimizing a clone and selecting a host system. In addition, engineering host cells to sustain expression for longer time periods will be discussed, along with overcoming cellular stress response to produce and secrete functionally active recombinant proteins.

 

Coverage will include, but is not limited to:

 

  • Recombinant DNA techniques
  • Changing a DNA sequence
  • Developing model systems
  • Codon optimization
  • Vector construction
  • CRISPR & gene editing tools
  • Genetic engineering
  • Selecting and optimizing clones
  • High-throughput cloning
  • Reformatting clones to an alternative vector
  • Efficient vector production
  • Characterizing the expressed protein
  • Determining amino acid sequences
  • Delineating protein shape and structure

 

* 活动内容有可能不事先告知作更动及调整。

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